High Performance Liquid Chromatographie Analysis o f Steroidal Saponins from Avena sativa L
نویسندگان
چکیده
High Performance Liquid Chromatography (HPLC), Steroidal Saponins, Avena sativa, Etioplasts Reversed phase high performance liquid chromatog raphy offers an efficient and rapid method for analysis of steroidal saponins. Crude extracts from primary leaves of Avena sativa and isolated etioplasts therefrom have been resolved into four saponins (avenacosides) using a wateracetonitrile gradient system on RP-8 and monitoring the column effluent at 200 nm with an UV-detector. Detectabil ity was found to be in the range of 50 ng avenacoside B and the detector response was linear up to 8 |ig tested. The described method is applicable to studies on localization and physiology of Avena saponins during development of the primary leaf.
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